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祝贺我司客户circRNA互作研究在毒理学权威杂志发表
人阅读 发布时间：2016-09-13 17:27
祝贺我司客户关于lncRNA、circRNA与miRNA三者互作的机制研究，目前被毒理学最具影响力杂志Archives of Toxicology收录，该杂志影响因子为6.637，其中RAP试剂盒、circRNA及lncRNA过表达载体和FISH探针由伯信生物提供，伯信生物将继续以科技创新为企业的发展动力和方向，成为广大科研伙伴的好助手。
LncRNA and circRNA expression was suppressed by siRNA-mediated knockdown. Three different siRNAs were designed and tested for both lncRpa and circRar1. Overexpression vectors for lncRpa and circRar1 were also constructed (BersinBio, Guangzhou, China).
Cells grown on coverslips were fixed with 4 % paraformaldehyde at room temperature for 15 min, washed twice with 0.1 % diethylpyrocarbonate solution and treated with 0.5 % Triton X-100 at room temperature for 5 min. The samples were dehydrated in a graded series of alcohol and air-dried.After adding probe hybridization solution, the samples were mounted, denatured at 73 °C for 3 min, and hybridized in a humid and dark environment at 37 °C for 12–16 h with Cy3-labeled miRNA probe, 6-carboxyfluoresceinlabeled circRNA probe, and Cy5-labeled lncRNA probe(BersinBio).
The RAP kit (BersinBio) was used for this experiment.RAP employs specific biotinylated probes that hybridize to target RNAs (mRNAs or miRNAs); these can then be pulled down, reverse transcribed to cDNA, and identified by qRT-PCR or sequencing.
目前公司拥有种类齐全的基因表达调控试剂盒：ChIP、reverse-CHIP、EMSA、RIP、RAP、DNA pull down、RNA pull down、荧光素酶报告基因、DNA甲基化、ChIRP试剂盒等。
已经为广大科研客户和合作伙伴提供了包括包括RNA干扰、qRT-PCR、southern-blot、northern-blot、ChIP、EMSA、reverse-CHIP、DNA/RNA pull down、FISH(荧光原位杂交)、稳定细胞系的构建、细胞永生化、甲基化、SNP、蛋白质表达及纯化、慢病毒载体构建及包装、腺病毒载体构建及包装、免疫组化、动物模型构建、SCI文章的修改及协助发表服务等等。
伯信生物本着诚信为本、科学严谨的服务理念为灵魂、坚实的技术服务基础为支撑、完善的售前售后服务为保障，以科技创新为企业的发展动力和方向，成为生命科学研究和人类健康领域产品及服务的提供者。
伯信生物，为您的科研工作提速!
文献链接：http://link.springer.com/article/10.1007%2Fs00204-016-1837-1
Materials and methods
RNA interference and overexpressionLncRNA and circRNA expression was suppressed by siRNA-mediated knockdown. Three different siRNAs were designed and tested for both lncRpa and circRar1. Overexpression vectors for lncRpa and circRar1 were also constructed (BersinBio, Guangzhou, China).
FISH
Cells grown on coverslips were fixed with 4 % paraformaldehyde at room temperature for 15 min, washed twice with 0.1 % diethylpyrocarbonate solution and treated with 0.5 % Triton X-100 at room temperature for 5 min. The samples were dehydrated in a graded series of alcohol and air-dried.After adding probe hybridization solution, the samples were mounted, denatured at 73 °C for 3 min, and hybridized in a humid and dark environment at 37 °C for 12–16 h with Cy3-labeled miRNA probe, 6-carboxyfluoresceinlabeled circRNA probe, and Cy5-labeled lncRNA probe(BersinBio).
RNA antisense purification (RAP)试剂盒
The RAP kit (BersinBio) was used for this experiment.RAP employs specific biotinylated probes that hybridize to target RNAs (mRNAs or miRNAs); these can then be pulled down, reverse transcribed to cDNA, and identified by qRT-PCR or sequencing.
目前公司拥有种类齐全的基因表达调控试剂盒：ChIP、reverse-CHIP、EMSA、RIP、RAP、DNA pull down、RNA pull down、荧光素酶报告基因、DNA甲基化、ChIRP试剂盒等。
已经为广大科研客户和合作伙伴提供了包括包括RNA干扰、qRT-PCR、southern-blot、northern-blot、ChIP、EMSA、reverse-CHIP、DNA/RNA pull down、FISH(荧光原位杂交)、稳定细胞系的构建、细胞永生化、甲基化、SNP、蛋白质表达及纯化、慢病毒载体构建及包装、腺病毒载体构建及包装、免疫组化、动物模型构建、SCI文章的修改及协助发表服务等等。
伯信生物本着诚信为本、科学严谨的服务理念为灵魂、坚实的技术服务基础为支撑、完善的售前售后服务为保障，以科技创新为企业的发展动力和方向，成为生命科学研究和人类健康领域产品及服务的提供者。
伯信生物，为您的科研工作提速!
文献链接：http://link.springer.com/article/10.1007%2Fs00204-016-1837-1
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